Bio-Inspired Molecularly Imprinted Polymer Electrochemical Sensor for Cortisol Detection Based on O-Phenylenediamine Optimization

This paper presents a comprehensive investigation of the various parameters involved in fabrication molecularly imprinted polymer (MIP) sensor for detection cortisol. Parameters such as monomer concentration, electropolymerization cycles, pH, monomer–template ratio, template removal technique, and rebinding time were optimized to establish more consistent effective method MIP sensors. Under conditions, demonstrated proportional decrease differential pulse voltammetry peak currents with increasing cortisol concentration range 0.1 100 nM. The exhibited excellent sensitivity, limit 0.036 Selectivity experiments using non-imprinted confirmed specific binding affinity cortisol, distinguishing it from other steroid hormones. study provides crucial insights into development reliable sensitive strategy O-PD-based MIPs. These findings laid foundation further advancements research.